T75 Cell Culture Flask: The Core Equipment in Cell Culture

Abstract:

T75 cell culture flask, also called T75 cell culture bottle, is a crucial device in cell culture. It is widely used in research and industry for the cultivation of cells, including primary cells, stem cells, and transformed cells. This article will discuss the T75 cell culture flask from six aspects, including its structure, materials, sterilization, cell culture techniques, applications, and maintenance.

Structure of T75 Cell Culture Flask

The T75 cell culture flask is a cylindrical container with a flat bottom and a screw cap. Its volume is 75 cm3, and its surface area is 75 cm2. The flask is made of high-quality polystyrene, which is transparent and has excellent optical properties. The flat bottom of the flask ensures the even distribution of cells, while the screw cap provides a tight seal to prevent contamination. The flask also has two vents to allow gas exchange during cell culture.

Materials of T75 Cell Culture Flask

The materials of the T75 cell culture flask are crucial for cell growth and viability. The polystyrene used in the flask is free of heavy metals and other toxic substances that may harm cells. The surface of the flask is treated with a special coating to enhance cell adhesion and proliferation. The coating may include collagen, fibronectin, laminin, or other extracellular matrix proteins. The flask also contains a filter to remove particles and bacteria from the culture medium.

Sterilization of T75 Cell Culture Flask

Sterilization is essential to prevent contamination and ensure the success of cell culture. The T75 cell culture flask can be sterilized by several methods, including autoclaving, gamma irradiation, or ethylene oxide gas. Autoclaving is the most common method and involves heating the flask to 121掳C for 15-20 minutes. Gamma irradiation is a non-thermal method that uses ionizing radiation to kill microorganisms. Ethylene oxide gas is a low-temperature sterilization method that uses a gas to penetrate the flask and kill microorganisms.

Cell Culture Techniques with T75 Cell Culture Flask

The T75 cell culture flask is suitable for a wide range of cell culture techniques, including adherent cell culture and suspension cell culture. Adherent cell culture involves the attachment of cells to the surface of the flask, while suspension cell culture involves the growth of cells in the culture medium without attachment. Adherent cell culture is commonly used for the cultivation of primary cells, while suspension cell culture is used for the cultivation of transformed cells and cell lines.

Applications of T75 Cell Culture Flask

The T75 cell culture flask has a wide range of applications in research and industry. It is used for the production of therapeutic proteins, vaccines, and monoclonal antibodies. It is also used for the study of cell biology, cancer research, and drug discovery. The flask is compatible with various cell types, including mammalian cells, insect cells, and plant cells.

Maintenance of T75 Cell Culture Flask

Proper maintenance of the T75 cell culture flask is crucial for the success of cell culture. The flask should be stored in a clean and dry environment to prevent contamination. The filter and cap should be checked for damage before use. The flask should be cleaned with a mild detergent and rinsed with sterile water before use. The culture medium should be changed regularly to maintain cell growth and viability.

Conclusion:

The T75 cell culture flask is a crucial device in cell culture. It provides a suitable environment for the growth and proliferation of cells and is compatible with various cell types. Proper use and maintenance of the flask are essential for the success of cell culture.